The former Friends star took the opportunity to show off her stunning honed figure, which defies the fact that she will turn 40 next year.
Aniston, who earlier this week gushed about her love for her 'extraordinary' boyfriend John Mayer, also fanned the flames over her rivalry with Angelina Jolie.
Revealing: Jennifer Aniston poses in nothing but a striped necktie in the forthcoming issue of American GQ
In a barbed remark, the actress joked that she goes on holiday with Jolie and Brad Pitt on weekends.
'The funny thing is that people don't realise we all go away to the Hamptons on the weekends,' she told the magazine.
'No. But can you imagine? That'd be hysterical,' and making a reference to the couple's children, she added: 'I've got Zahara on my hip, and Knox....'
She also spoke about how she is still in touch with Pitt.
'We don't not talk,' she said. 'When there's something to congratulate or celebrate, there's always an exchange. But there's no charge on it.'
And asked if she talks to Jolie, she retorted: 'No, Nuh-uh.'
Aniston's latest rebuttal comes as she faced the final insult this week over her marriage breakdown with Pitt after he reiterated Jolie's recent confession that they fell in love while filming Mr and Mrs Smith in 2004 - seven months before he split with Aniston.
'Extraordinary': Aniston with her 31-year-old lover, singer John MayerAfter being asked why the action film is his favourite, he explained: 'Because you know... six kids. Because I fell in love.'
His revelation came two months after Jolie told the New York Times that she fell for him while making the movie.
Referring to their children watching the film, she said: 'Not a lot of people get to see a movie where their parents fell in love.'
The GQ front cover from 2005
In an interview with the Daily Mirror, Aniston said: 'He's a rare one. He is extraordinary and it is wonderful to watch him.
'The way his brain works and the way he thinks thoughts... it's beautiful.
'It's an amazing thing to watch a musician think. I don't know many musicians but when his guitar is on him it's just like a channel.
'It's something I've never ever seen before.'
The last time the star graced the front cover of the magazine was in 2005 when she appeared in nothing but a short denim skirt.
A full version of the interview appears in American GQ, available from December 23.